In particular, polystyrene eNPs have an extensive use as systems for in vitro applications such as cell-sorting, labelling and cellular delivery 16, 17. Therefore, Engineered Nanoparticles (eNPs) are increasing their relevance in various fields, including targeted drug and gene delivery systems, biosensors and cancer treatment 6, 7, 8, 9, 10, 11, 12, 13, 14, 15. Nanoparticles (NPs) from a wide range of different organic and inorganic materials are used in different biological and medical applications, such as diagnostic, non-invasive imaging and drug delivery to mention just a few 2, 3, 4, 5. Over the last decades, nanotechnologies have become essential tools in the biomedical field with a large number of products based on nanoparticles being found in the market 1.
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